Protocols

The following protocols are available

ES Cell Preparation, Transfection & Preservation

Thawing MEF cells, expansion and inactivation
Thawing and feeding ES cells
Electroporation
Feeding transfected ES cells in Selection Media
Picking ES clones
Disaggregation, expansion and freezing of transfected ES clones
Expansion of your targeted ES clones
Transient transfection protocol for pTurbo-cre
Murine Tissue Protocols

Genomic DNA preparation from ES cells
Mouse Tail (or organ) Biopsy DNA Extraction
Preparation of Murine Embryonic Fibroblast (MEF) feeder cells
Recommended reagent sources and recipes
Reporter Assay Protocols

β-gal Assay
ONPG Assay
DCM MAP/RAP test & protocol