Dear Investigator:

We are pleased to announce the continuation of mouse embryonic stem (ES) cell services for the WUSM research community. Effective November 1, 2014, the Washington University Mouse ES Core will be under the combined leadership of Drs. Tim Ley, David Ornitz, and Jeff Miner. All services previously provided by the Siteman ES Core will be available in the new Core. We do not anticipate any disruption in continuity: the new Core will open on November 1, 2014.

This continuance was made possible by generous support from the Departments of Medicine and Developmental Biology, with additional support from the Departments of Ophthalmology, Cell Biology, and Orthopedic Surgery. We anticipate that additional Departments will also participate to ensure the stability of ES services here.

The ES Core will be moving to room #377 of the McDonnell Science Building in the near future. This new lab space has been graciously provided to the ES Core by the Department of Developmental Biology.

We would like to thank everyone for their additional support in terms of letters and e-mails. A group effort truly made this possible.

Best regards,

Jacqueline L. Mudd | Dr. Tim Ley | Dr. David Ornitz | Dr. Jeff Miner

Transfected Cell Line Performance Data

(1997 to present)


The Washington University Mouse Embryonic Stem Cell (ESC) Core is a service generously supported by the Departments of Medicine, Developmental Biology, Cell Biology, Ophthalmology, and Orthopedic Surgery. The mission of the ESC Core is to help you create mutations in murine embryonic stem cells. The core has several aspects to this mission, including development of state-of-the-art reagents for the production of targeted mutations in embryonic stem cells, the creation of quality-controlled embryonic stem cell lines, and the teaching of methods for embryonic stem cell culture and manipulation. The core provides quality-controlled cells developed here at Washington University. In addition, our core can provide a teaching service, so that "hands-on" production of targeted ES clones can be learned. Finally, the core will attempt to assist all users with blastocyst injection, if injection services are not available to that laboratory. By providing a comprehensive service, we hope to facilitate the production of gain-of-function and loss-of-function mouse models for our faculty.

The core will be accepting non-WUSM work, and has a price structure that allows other institutions, non-profits and commercial interests to be able to participate. These charges are detailed on the Services page.

Publication Information

As of April 7, 2008, the NIH requires investigators with a publication using any NIH-funded shared resources to submit (or have submitted for them) their final, peer reviewed manuscripts to PubMed Central(PMC) upon acceptance of publication, to be made publicly available within 12 months of publication. Many journals automatically submit these for authors, but Washington University also has assistance available through the Becker Medical Library. Please see or for more information.

PUBLICATION ACKNOWLEDGEMENT: If research supported by the Embryonic Stem Cell Core results in publication, please acknowledge this support by including the following in your publication(s):

We thank the Washington University at St. Louis for the use of the Embryonic Stem Cell Core, which provided __________ service.

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Site Updated: November 05, 2014