Services Offered
The ES cell line RW.4, formerly available through the Core, is now being distributed by the American Type Culture Collection. The analysis information specific to this cell line is linked here.
B6/BLU; EDJ-22; SCC-10, B6-GFP Murine Stem Cells
Established quality controlled embryonic stem cell lines (B6/BLU; EDJ-22; SCC-10; B6-GFP) are available to the Washington University Community only. These ES lines are highly efficient and have proven germline capabilities. All Washington University investigators are allowed to purchase up to 6 vials per year for their laboratories.
One vial of our ES cells will provide enough ES cells for one electroporation by our protocol. If these vials are expanded further in your laboratory for further electroporations, we cannot guarantee their ability to go germline. To order a vial of our ES cells from the ES Core, please direct a FAX to us at 362-4298 or E-mail your request to Elaine Ross or Jacqueline Mudd, Email: escore@im.wustl.edu Please leave your phone number or E-mail address so we can contact you with a good time for you to pick up your cells.
Cost: $250.00 per vial
- Expansion of Targeted ES clones
Cost: $100 per clone expanded (Maximum expansion of 10 clones).
- Training
3 full time senior technicians will provide instruction in the following techniques:
- Transfection of targeting constructs into embryonic stem cell lines
- Selection of targeted clones
- Picking and expansion of clones (up to 144)
- Cryopreservation and generation of cells for identification of homologous recombinants
- Transfection of targeting constructs into embryonic stem cell lines
- WUMS Microinjection Service Options
- Mouse Genetics Core
http://mgc.wustl.edu - Pathology Microinjection Core
http://pathology.wustl.edu - Ron McCarthy
RMcCarth@im.wustl.edu
Tel: 314 454-7990
- Mouse Genetics Core
- Plasmids available
- Lox P flanked pgk-neo cassette:
p # 1338 and p # 1339
- cre-recombinase expression plasmid: pTurbo-cre
(requires a Material Transfer Agreement for non-Washington University researchers)
- Lox P flanked pgk-neo cassette:
- Transfections
- Standard transfections - $1,800.00 (for up to 144 picked clones)
- Cre transfections
- - $1,000.00 (if 48 clones are picked)
- - $1,800.00 (if 144 clones are picked)
After receipt of your application for an electroporation you will be advised of its scheduled date. We will require 40ug of LINEARIZED DNA at a concentration of 1 ug/ul in TE Buffer for this electroporation. We would like to have documentation at least one week prior to the scheduled electroporation in the form of a gel photograph of what is considered to be 1 ug of the sample DNA intended for use for transfection. Elaine Ross or Jacqueline Mudd will contact you to arrange your placement on the schedule.
- ES Cell Line Derivations
Requirements from the WUMS investigator are detailed in the "Protocols" section.
Cost to Investigator:
$ 5000.00 base
- The ES core will require an initial base fee of $2500.00 when the work begins and the final base fee of $2500.00 upon completion of the work.
- The ES core cannot provide any guarantees.
+ $ 1000.00 per each primary line expanded
Additional Costs to Investigator:
MAP test = $300.00 - $320.00 per line (DCM charge)
DCM Contact: Dr. Suellen Greco, tel. 314-362-3125Mycoplasma test = $58.70 for 1 - 3 lines
= $53.05 for 4 - 5 lines
= $49.95 for 6 lines or more (WUTCC charge)Karyotyping = $350.00 per line (Dr. Chih-Lin Hsieh charge / see below)
- The ES core will require an initial base fee of $2500.00 when the work begins and the final base fee of $2500.00 upon completion of the work.
- Karyotyping Services
Arrangements for ES cell karyotyping may be made by contacting Dr. Chih-Lin Hsieh.
E-mail: hsieh_c@ccnt.hsc.usc.eduKaryotyping services are also provided by:
Dr. Shashikant Kulkarni
Cytogenomics Core Facility
Washington University School of Medicine
Campus Box 8116
660 South Euclid
St. Louis, MO 63110
Ph: (314) 454-8418
Fax: (314) 454-5192
The ES Core does not provide Murine Embryonic Fibroblasts for sale to the Washington University Community. However, you may purchase them through Specialty Media.(Tel. 888-209-8870, Web: www.specialtymedia.com ). We do provide the protocol for the preparation of feeder layers from Primary Embryonic Fibroblasts on our Protocols page. Also provided is the ordering information for obtaining the breeding mice from the Jackson Laboratories.