Protocol Menu Thawing MEF cells, expansion and inactivation Thawing and feeding ES cells Electroporation Feeding transfected ES cells in Selection Media Picking ES clones Disaggregation, expansion and freezing of transfected ES clones Expansion of your targeted ES clones Transient transfection protocol for pTurbo-cre Genomic DNA preparation from ES cells Mouse Tail (or organ) Biopsy DNA Extraction Preparation of Murine Embryonic Fibroblast (MEF) feeder cells Recommended reagent sources and recipes β-gal Assay ONPG Assay DCM MAP/RAP test and protocol. ACK Lysis protocol. ES Cell Line Derivations. Click on the calendar day to review these daily protocol instructions, or alternatively go to the download center to retrieve a full copy as a file.
Click on the calendar day to review these daily protocol instructions, or alternatively go to the download center to retrieve a full copy as a file.