UPDATE: Effective November 1, 2014, the core will be renamed the Washington University Mouse Embryonic Stem Cell (ESC) Core. The ESC Core will be supported by the Departments of Medicine, Developmental Biology, Cell Biology, Ophthalmology, and Orthopedic Surgery and continue to provide support to the Washington University community.


The Murine Embryonic Stem Cell Core has been created to help you create mutations in murine embryonic stem cells. The core has several missions, including development of state-of-the-art reagents for the production of targeted mutations in embryonic stem cells, the creation of quality-controlled embryonic stem cell lines, and the teaching of methods for embryonic stem cell culture and manipulation. The core provides quality-controlled cells developed here at Washington University. In addition, our core can provide a teaching service, so that "hands-on" production of targeted ES clones can be learned. Finally, the core will attempt to assist all users with blastocyst injection, if injection services are not available to that laboratory. By providing a comprehensive service, we hope to facilitate the production of gain-of-function and loss-of-function mouse models for our faculty.


We are receiving ES clones from the KOMP repository at UC-Davis (as well as EUCOMM & TIGM). Click on the diagram for more detailed information. This will generate a new window.

Important Information concerning murine C57BL6N substrains

 All C57BL/6N mice, regardless of vendor, are homozygous for the rd8 retinal degeneration mutation in the Crb1 gene. This is a recent finding relevant to Ophthalmology researchers in particular, yet it brings attention to the possibility of false phenotypes due to genome variability within the C57BL/6 mouse substrain.


Publication Information

As of April 7, 2008, the NIH requires investigators with a publication using Siteman (or other NIH-funded) shared resources to submit (or have submitted for them) their final, peer reviewed manuscripts to PubMed Central(PMC) upon acceptance of publication, to be made publicly available within 12 months of publication. Many journals automatically submit these for authors, but Washington University also has assistance available through the Becker Medical Library. Please see http://publicaccess.nih.gov/FAQ.htm#b7 or http://becker.wustl.edu/services/scholarly/nihpolicy.html for more information.

PUBLICATION ACKNOWLEDGEMENT: If research supported by the Embryonic Stem Cell Core results in publication, please acknowledge this support by including the following in your publication(s):

We thank the Alvin J. Siteman Cancer Center at Washington University School of Medicine and Barnes-Jewish Hospital in St. Louis, Mo., for the use of the Embryonic Stem Cell Core, which provided __________ service. The Siteman Cancer Center is supported in part by an NCI Cancer Center Support Grant #P30 CA91842.

Disclaimer and Copyright

Murine Embryonic Stem Cell Core

The Alvin J. Siteman Cancer Center at Washington University

St. Louis, Missouri 63110

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Site Updated: Sep 29, 2014